Journal of Engineering and Engineering Technology - JEET |DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF CLINICAL ISOLATES FROM A HOSPITAL IN SOUTH -SOUTH NIGERIA

Paper Details

Title :DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF CLINICAL ISOLATES FROM A HOSPITAL IN SOUTH -SOUTH NIGERIA
Author(s) : Clement Ndudi Isibor (1) and Johnson .D Jemikalajah (2)
DOI:
Abstract:

Infections caused by microorganisms have become a significant public health concern in Nigeria, leading to increased morbidity and mortality rates. This study was designed to evaluate the in-vitro activities of three commonly prescribed quinolone and cephalosporin agents against clinical bacterial isolates from Central Hospital Warri. The minimum inhibitory concentrations (MIC) were investigated using the agar dilution method. The invitro activity of quinolones- pefloxacin, ciprofloxacin, ofloxacin were compared with 3 other agents namely ceftazidime, cefuroxime, and gentamicin. Pefloxacin showed greater activity followed by ofloxacin and ciprofloxacin. Overall, the MIC90 values for the quinolones, ciprofloxacin, pefloxacin, and ofloxacin were below 2 mg/l. Pefloxacin and ofloxacin inhibited 90% of isolates of Escherichia coli, Klebsiella sp, Staphylococcus aureus and Proteus sp at values <0.6mg/l. The MIC90 value for ciprofloxacin was higher with 1.76 mg/l as MIC90, for Klebsiella sp in this study. Against Pseudomonas aeruginosa, ofloxacin had MIC90 values of 2.75 mg/l. The MIC90 for Staphylococcus aureus was 0.5, 0.7 and 1.2 mg/l respectively for pefloxacin, ofloxacin and ciprofloxacin. Pefloxacin was therefore adjudged the most active of the quinolone compounds in this study. However, the in-vitro potency of pefloxacin was equivalent to that of ofloxacin which could be prescribed as an alternative, but greater than those of cefuroxime, ceftazidime, and gentamicin. It is therefore pertinent to state that medical laboratories should periodically review their antibiotic usage to curb the resistant issues among organisms.

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